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Cortisol 4 Point test kit
Saliva collection close-up
Laboratory work scene
Functional Health • Saliva based
Cortisol

Cortisol 4 Point

The SimplyTest® Cortisol 4 Point is a noninvasive salivary cortisol assessment that measures active, free cortisol at four points throughout the day to evaluate diurnal rhythm and HPA-axis function.

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Key features

  • Non-invasive and simple to collect (home or clinical setting).
  • Measures “free” cortisol (biologically active fraction).
  • Four timepoints to understand the full diurnal pattern.
  • Convenient multi-sample collection with minimal disruption.
Cortisol 4 Point Fact Sheet Provider Fact Sheet View Sample Report

Understand daily stress rhythm—beyond a single snapshot.

Cortisol naturally fluctuates throughout the day in a diurnal pattern. Measuring cortisol at four points helps reveal whether the curve is elevated, blunted, shifted, or imbalanced—insights that can support fatigue, sleep, mood, and metabolic conversations.

Salivary cortisol levels have been shown to correlate with serum levels, providing comparable clinical insight through a more patient-friendly, noninvasive method.

Download Cortisol 4 Point Fact Sheet
Collect
4 saliva samples
Collected at key points during one day to map the diurnal rhythm.
Measure
Active “free” cortisol
Saliva reflects the biologically active form unbound to proteins.
Interpret
Rhythm & patterns
Supports evaluation of stress response, HPA-axis function, and metabolic balance.

What Cortisol 4 Point measures

Cortisol is measured at four points throughout the day to assess the diurnal curve—helping identify timing shifts (high at night, low in the morning, flat patterns, etc.) that may not be visible with a single draw.

If you want your exact collection times listed (e.g., “30 minutes after waking”), tell me your preferred timing language and I’ll update the pills to match your kit instructions.

4-point timeline
  • Point 1Morning
  • Point 2Midday
  • Point 3Late afternoon
  • Point 4Evening
Measures free cortisol at 4 daily points to evaluate diurnal rhythm.

When to test

Consider Cortisol 4 Point when you want to better understand stress rhythm and daily cortisol patterning.

  • Fatigue, low resilience, or “burnout” concerns.
  • Sleep disruption, especially trouble falling or staying asleep.
  • Stress-related symptoms (mood, motivation, irritability).
  • Metabolic / weight concerns and blood sugar regulation conversations.
  • Monitoring stress-response changes over time (lifestyle or therapy support).
  • Patients who need noninvasive, at-home-friendly collection.

How it works

A simple collection and reporting flow built for convenience and consistency.

1

Collect at 4 points

Collect saliva samples at the designated times across a single day.

2

Ship to the lab

Package the samples using the included materials and send to the lab.

3

Review the pattern

Access results in the SimplyTest Provider Portal and apply to care planning.